The physical position of Pvr9 in the pepper genome was predicted by nucleotide blasting against chromosome data sets of the pepper genome database (http://cab.pepper.snu.ac.kr/). The highest homologous sequences (>99%) of 4041 CCCP pairs belonged to chromosome 6 in pepper C. annuum Zunla-1 and C. annuum var. grabriusculum. They were determined to be located from nucleotide 213,329,659 to nucleotide 213,326,619 of C. annuum Zunla-1 chromosome 6 and from nucleotide 195,062,197 to nucleotide 195,058,157 of C. annuum var. grabriusculum chromosome 6. In Fig. 1C, the position of Pvr9 on pepper chromosome 6 is schematically represented relative to the previously published markers C2At3g25120, C2At2g39690, C2At3g46780, and CVMV-3 ( Lee et al., 2013 and Wu et al., 2009).
Elicitor identification and characterization
To determine which PepMoV gene triggers the hypersensitive response mediated by Pvr9, we cloned the viral genes from PepMoV isolate 134 (Fig. 2A) (Kim et al., 2009) in the modified vector pPZP212 and transformed the cloned genes into Agrobacterium. The transformants of individual viral gene and Pvr9 were then co-infiltrated into N. benthamiana. As shown in Fig. 2B, co-agroinfiltration of transformants expressing NIb and Pvr9 triggered a hypersensitive response but co-infiltration of the other transformants did not. This demonstrated that PepMoV NIb is the elicitor of the hypersensitive response. However, direct interaction between the NIb and Pvr9 was not found in yeast two hybrid and bimolecular fluorescent complementation assay ( Supplementary Fig. S1).
Elicitor identification and characterization
To determine which PepMoV gene triggers the hypersensitive response mediated by Pvr9, we cloned the viral genes from PepMoV isolate 134 (Fig. 2A) (Kim et al., 2009) in the modified vector pPZP212 and transformed the cloned genes into Agrobacterium. The transformants of individual viral gene and Pvr9 were then co-infiltrated into N. benthamiana. As shown in Fig. 2B, co-agroinfiltration of transformants expressing NIb and Pvr9 triggered a hypersensitive response but co-infiltration of the other transformants did not. This demonstrated that PepMoV NIb is the elicitor of the hypersensitive response. However, direct interaction between the NIb and Pvr9 was not found in yeast two hybrid and bimolecular fluorescent complementation assay ( Supplementary Fig. S1).