Following inoculation, leaf stunting was evident at 7 dpi (days after inoculation) and yellowing was evident at 15 dpi in Floral Gem but not in CM334 (Fig. 5A). The qRT-PCR signals representing PepMoV CP transcripts increased in Floral Gem but not in CM334 (Fig. 5B). In Floral Gem, the signal logarithmically increased in inoculated (local) Icotinib and drastically increased in upper leaves (systemic) after 5 dpi. However, the signal decreased in both local and systemic leaves of CM334 (except for a slight increase in local leaves by 3 dpi). Pvr9 transcript levels were generally stable in the upper leaves of both pepper cultivars ( Fig. 5C and D, line graphs). In virus-inoculated leaves of CM334, the Pvr9 transcript level increased slightly from 2 to 10 dpi in comparison to mock inoculation (Fig. 5C, white and grey bars). In Floral Gem, however, Pvr9 transcription was not induced by PepMoV inoculation; at some time points (1, 3, 10, and 15 dpi), the transcript levels were lower in PepMoV-inoculated leaves than in mock-inoculated leaves (Fig. 5D, white and grey bars).
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