Fig. 3. Strategy for construction of recombinant bacmids. This schematic shows the construction strategy for bacmids used in this study. The bacmid names are shown above the diagrams. Open arrows indicate genes and promoters (pr) and the direction of transcription. Loci where a modification was introduced is shown in the rectangle above or below the I-BET151 cassettes. egfp, enhanced green fluorescent protein gene; polh, polyhedrin; zeo, zeocin.Figure optionsDownload full-size imageDownload high-quality image (265 K)Download as PowerPoint slide
Sf9 cells were infected with Ac-CpGV-ORF46-PG and production of CpGV-MMP over the course of infection was monitored by immunoblotting using an anti-HA antibody. An HA-immunoreactive band was observed in immunoblots from pellet and extracellular media lysates derived from Ac-CpGV-ORF46-PG-infected cells (Fig. 4A). Detection of CpGV-MMP in the extracellular media confirmed secretion of CpGV-MMP. An immunoreactive protein was not detected in mock- or AcWT-PG-infected Sf9 cells collected at 12 h postinfection (p.i.) (Fig. 4A).
Sf9 cells were infected with Ac-CpGV-ORF46-PG and production of CpGV-MMP over the course of infection was monitored by immunoblotting using an anti-HA antibody. An HA-immunoreactive band was observed in immunoblots from pellet and extracellular media lysates derived from Ac-CpGV-ORF46-PG-infected cells (Fig. 4A). Detection of CpGV-MMP in the extracellular media confirmed secretion of CpGV-MMP. An immunoreactive protein was not detected in mock- or AcWT-PG-infected Sf9 cells collected at 12 h postinfection (p.i.) (Fig. 4A).