2.6.5. Encapsulation efficiency (EE)
Encapsulation efficiency (Equation (5)) was expressed as the difference between total and superficial gallic SNDX-275 concentrations, in relation to the amount of gallic acid added in the emulsions before atomization in spray chilling.equation(5)EE(%)=(TGA−SGAEGA)∗100in which TGA, SGA and EGA are the total, superficial and emulsion gallic acid concentrations.
2.6.6. Microstructure
Morphology of lipid microparticles was evaluated in a Scanning Electron Microscope (SEM) (LEO Electron Microscopy, model Leo 440i, Oxford–Cambridge, England) with an energy dispersive X-ray (EDX) detector. Acceleration voltage equal to 5 kV and beam current of 50 pA were used. Before analysis, microparticles were covered with a gold layer by a Sputter Coater Polaron (VG Microtech, model SC7620, Uckfield, England).
2.7. Statistical analysis
Data were statistically analyzed by Tukey test using the Statistic 12 software (Statsoft, Tulsa, Okla., USA). Differences between means were considered significant at a 95% confidence level (p ≤ 0.05).
Encapsulation efficiency (Equation (5)) was expressed as the difference between total and superficial gallic SNDX-275 concentrations, in relation to the amount of gallic acid added in the emulsions before atomization in spray chilling.equation(5)EE(%)=(TGA−SGAEGA)∗100in which TGA, SGA and EGA are the total, superficial and emulsion gallic acid concentrations.
2.6.6. Microstructure
Morphology of lipid microparticles was evaluated in a Scanning Electron Microscope (SEM) (LEO Electron Microscopy, model Leo 440i, Oxford–Cambridge, England) with an energy dispersive X-ray (EDX) detector. Acceleration voltage equal to 5 kV and beam current of 50 pA were used. Before analysis, microparticles were covered with a gold layer by a Sputter Coater Polaron (VG Microtech, model SC7620, Uckfield, England).
2.7. Statistical analysis
Data were statistically analyzed by Tukey test using the Statistic 12 software (Statsoft, Tulsa, Okla., USA). Differences between means were considered significant at a 95% confidence level (p ≤ 0.05).