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The formation of Ag/TiO2/CS nanocomposite was further evidenced by XRD analysis. Fig. 5 shows the XRD patterns of AgNO3, TiO2, chitosan, and Ag/TiO2/CS nanocomposite. The characteristic peaks of the raw materials were generated at 35.5° (AgNO3), 25.3° (TiO2), and 19.7° (chitosan). These peaks suggest the highly crystalline nature of these GNE-9605 three compounds, which is consistent with existing literature (Au et?al., 2012, Murugadoss and Chattopadhyay, 2008 and Qi et?al., 2004). These peaks are also observed in the XRD profile of nanocomposite, although with significantly lower intensities and minor shift to 38.4° (Ag), 21.6° (TiO2), and 18.7° (chitosan). In addition, the peak corresponding to chitosan in the nanocomposite was wider than heterogametic sex in the profile for pure chitosan. These results indicated the successful incorporation of silver in the nanoscale CS matrix, which takes place mainly in the amorphous region of CS (Modrzejewska, Binias, Wojtasz-Paja?k, Dorabialska, & Zarzycki, 2008).

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