The occurrence of spindles in the LFP showed a similar pattern to that of slow waves, with a significant diurnal effect for striatal (F(3,28) = 4.93, p < 0.01), but not cerebellar recordings (F(3,12)
= 2.12, p = 0.15) (Figure 鈥�(Figure5B).5B). The diurnal pattern of spindles was dependent upon the occurrence of slow waves, such that when normalized to the amount of time spent in 0鈥�3 Hz oscillations there was no diurnal variation in spindle incidence (Str, F(3,28) = 1.16, p = 0.34; CB, F(3,12) = 1.54, p = 0.25) (Figure 鈥�(Figure5C).5C). In addition, raclopride did not have an effect on the incidence of spindles (Str, F(3,28) = 1.17, p = 0.34; CB, F(3,12) = 1.88, p = 0.20; data not shown). Therefore, diurnal variations in the incidence of spindles are dependent on the presence of slow wave oscillations under our urethane anesthesia preparation, and are not significantly affected www.selleckchem.com/products/otx015.html by D2 receptor antagonism. Effects Bilirubin oxidase of raclopride on diurnal variations in coherence To assess the overall interregional
differences in the synchrony of LFP oscillations recorded in the striatum and cerebellum, coherence within and between these structures was evaluated. Within the 0鈥�3 Hz and 3鈥�8 Hz bands, coherence showed similar levels and were therefore combined. There was also no difference between the following comparisons, so this data was combined: MStr and LStr within pair, the MStr-CB and LStr-CB, the two cortical locations with the CB, and the two
off-set cortico-striatal comparisons. 0鈥�8 Hz coherence followed a similar pattern as 8鈥�55 Hz coherence, but was consistently higher (see Figure 鈥婩igure6A,6A, 0鈥�8 Hz vs. 0鈥�55 Hz). Coherence was higher for recordings obtained from adjacent tips within each of the striatal electrode pairs (Within Pair) as compared to recordings obtained between electrode pairs (Btwn Pair) in the selleck chemicals llc MStr vs. LStr (p < 0.01) or across electrode pairs in the cerebellum (p < 0.001). This demonstrates enhanced coherence of LFPs with spatial proximity in both the striatum and the cerebellar cortex (Figure 鈥�(Figure6A,6A, Within Pair vs. Btwn Pair comparisons). Four additional recording sessions were performed to evaluate the contribution of the neocortex to the striato-cerebellar coherence and included simultaneous recordings from two striatal electrodes, two electrodes in the overlying neocortex, and four electrodes in the cerebellar cortex. Cortico-striatal coherence was greatest between electrodes in the same sagittal plane and was comparable to the striatal-within pair comparisons (Figure 鈥�(Figure6A,6A, 0鈥�8 Hz: Str-within pair = 0.92 卤 0.024; Ctx-Str vertical = 0.90 卤 0.042). Cortico-striatal coherence was reduced for comparisons in different medial vs. lateral positions, showing a similar coherence as the striatal-between pair comparisons (Str-between pair = 0.82 卤 0.016; Ctx-Str off-set = 0.79 卤 0.042).
= 2.12, p = 0.15) (Figure 鈥�(Figure5B).5B). The diurnal pattern of spindles was dependent upon the occurrence of slow waves, such that when normalized to the amount of time spent in 0鈥�3 Hz oscillations there was no diurnal variation in spindle incidence (Str, F(3,28) = 1.16, p = 0.34; CB, F(3,12) = 1.54, p = 0.25) (Figure 鈥�(Figure5C).5C). In addition, raclopride did not have an effect on the incidence of spindles (Str, F(3,28) = 1.17, p = 0.34; CB, F(3,12) = 1.88, p = 0.20; data not shown). Therefore, diurnal variations in the incidence of spindles are dependent on the presence of slow wave oscillations under our urethane anesthesia preparation, and are not significantly affected www.selleckchem.com/products/otx015.html by D2 receptor antagonism. Effects Bilirubin oxidase of raclopride on diurnal variations in coherence To assess the overall interregional
differences in the synchrony of LFP oscillations recorded in the striatum and cerebellum, coherence within and between these structures was evaluated. Within the 0鈥�3 Hz and 3鈥�8 Hz bands, coherence showed similar levels and were therefore combined. There was also no difference between the following comparisons, so this data was combined: MStr and LStr within pair, the MStr-CB and LStr-CB, the two cortical locations with the CB, and the two
off-set cortico-striatal comparisons. 0鈥�8 Hz coherence followed a similar pattern as 8鈥�55 Hz coherence, but was consistently higher (see Figure 鈥婩igure6A,6A, 0鈥�8 Hz vs. 0鈥�55 Hz). Coherence was higher for recordings obtained from adjacent tips within each of the striatal electrode pairs (Within Pair) as compared to recordings obtained between electrode pairs (Btwn Pair) in the selleck chemicals llc MStr vs. LStr (p < 0.01) or across electrode pairs in the cerebellum (p < 0.001). This demonstrates enhanced coherence of LFPs with spatial proximity in both the striatum and the cerebellar cortex (Figure 鈥�(Figure6A,6A, Within Pair vs. Btwn Pair comparisons). Four additional recording sessions were performed to evaluate the contribution of the neocortex to the striato-cerebellar coherence and included simultaneous recordings from two striatal electrodes, two electrodes in the overlying neocortex, and four electrodes in the cerebellar cortex. Cortico-striatal coherence was greatest between electrodes in the same sagittal plane and was comparable to the striatal-within pair comparisons (Figure 鈥�(Figure6A,6A, 0鈥�8 Hz: Str-within pair = 0.92 卤 0.024; Ctx-Str vertical = 0.90 卤 0.042). Cortico-striatal coherence was reduced for comparisons in different medial vs. lateral positions, showing a similar coherence as the striatal-between pair comparisons (Str-between pair = 0.82 卤 0.016; Ctx-Str off-set = 0.79 卤 0.042).