Fig. 5. IRF-7 and NF-κB are involved in controlling the production of IFNs. (A and C) 293T G007-LK stably expressing specific shRNA targeting IRF-7 or luciferase control were infected with or without MDRV for 24 h. The expression of IFN-β, IL-28A/B and IL-29 was analyzed by RT-PCR (A) and quantitative real-time PCR (C). (B) The interference efficiency of IRF-7 was verified by quantitative real-time PCR. (D) 293T cells stably expressing specific shRNA targeting IRF-3 or luciferase control were treated as described in (A). The expression of indicated genes was analyzed by RT-PCR. (E) Liver tissues of Muscovy ducks infected with MDRV for the indicated times were used to detect the protein expression of IκB-α by Western blotting. The results are representative of three independent experiments. (F and G) 293T cells were treated with BAY11-7082 (3 μM) or DMSO for 2 h before infection with MDRV. After 24 h infection, the cells were harvested and the mRNA levels of IFN-β, IL-28A/B and IL-29 were analyzed by RT-PCR (F) and quantitative real-time PCR (G). Plotted are the average results from three independent experiments. *P < 0.05.Figure optionsDownload full-size imageDownload as PowerPoint slide
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